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Quantitative PCR was performed with PowerUp SYBR Green Master Mix (Thermo Fisher Scientific, Waltham, MA, USA) on a Mastercycler nexus X2 (Eppendorf, Hamburg, Germany)

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92 On the other hand, protein phosphatase 2A (PP2A) is the major dephosphorylating enzyme, along with other phosphatases (e.g., PP1, PP5, and PP2B)